US 2022/0010315 A1 · METHOD FOR PRODUCING WASHING ENZYME HAVING PROTEASE RESISTANCE

Abstract

Provided is a method for producing washing enzyme having protease resistance. According to the method, the washing enzyme having resistance to protease is obtained by carrying out fusion expression on a gene of the washing enzyme with the gene of a protease inhibitory peptide, thereby facilitating maintaining the stability of various enzyme components in an enzyme-containing detergent, and improving the use effect of the detergent.

Claims10

Dependent claims

A method for producing a laundry enzyme with protease resistance, comprising:

(a) constructing a fusion polynucleotide sequence comprising a gene encoding a laundry enzyme and a gene encoding a protease inhibitory peptide;
(b) introducing the sequence into a host cell;
(c) culturing the host cell, expressing the sequence and producing a fusion protein of laundry enzyme-protease inhibitory peptide, wherein the fusion protein is the laundry enzyme with protease resistance.

Dependencies are inferred from the claim text.

Facts

Application

17/288,530Filed 28 Oct 2019

Priority

CN 201811285860.931 Oct 2018

Published

13 Jan 2022

Term ends

Not estimatedThe source records don’t support an estimate.

Classification14 codes

C12N 15/62
DNA or RNA fragments; Modified forms thereof ; Non-coding nucleic acids having a biological activity: dNA sequences coding for fusion proteins
C07K 14/81
Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof: protease inhibitors
C07K 14/811
Endopeptidase inhibitors: serine protease inhibitors
C12N 9/20
Carboxylic ester hydrolases: triglyceride splitting, e.g. by means of lipase

Method for producing washing enzyme having protease resistance

Life of this patent

Method for producing washing enzyme having protease resistance

Life of this patent

Abstract

Claims10