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US 2022/0010299 A1Published application
Systems and Methods to Enhance RNA Stability and Translation and Uses Thereof
AssigneeThe Board of Trustees of the Leland Stanford Junior University
InventorsRhiju Das, Christian A. Choe, Hannah K. Wayment-Steele, Wipapat Kladwang
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Abstract
Embodiments herein describe systems and methods to enhance RNA translation and stability and uses thereof. Many embodiments generate RNA molecules possessing increased structure and/or reduced free energy over an initial sequence. Such RNA molecules can be used as therapeutics and/or vaccines.
Claims30
An RNA therapeutic comprising:
- an RNA molecule comprising a 5′ untranslated region, a 3′ untranslated region, and a coding sequence;
- wherein the 5′ untranslated region is located 5′ of the coding sequence and the 3′ untranslated region is located 3′ of the coding sequence, and
- wherein the coding sequence encodes for one or more viral epitopes.
A method for increasing RNA stability comprising:
- obtaining a target RNA sequence comprising a coding sequence;
- altering at least one nucleotide within the RNA sequence, wherein the altered sequence improves a metric correlated with improved RNA function;
- and
- synthesizing an RNA molecule representing the altered sequence.
1 more element
An RNA molecule to transfect a cell comprising:
- a 5′ untranslated region, a 3′ untranslated region, and a coding sequence, wherein the 5′ untranslated region is located 5′ of the coding sequence and the 3′ untranslated region is located 3′ of the coding sequence.
Dependencies are inferred from the claim text.