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US 2022/0010288 A1Published application
Protein-based purification matrices and methods of using the same
AssigneeIsolere Bio, Inc.
InventorsKelli M. LUGINBUHL, Michael DZURICKY
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Abstract
Provided herein are protein-based purification matrices and methods of use thereof to purify biologics and/or to remove contaminants from a composition. Methods of bringing two or more biologics in close proximity are also provided. The disclosed compositions and methods allow for faster, more efficient purification of a biologic compared to traditional affinity chromatography.
Claims28
A protein-based purification matrix comprising a fusion protein, wherein the fusion protein comprises:
- (i) at least one retrovirus- or lentivirus-binding domain;
- and
- (ii) at least one polypeptide with phase behavior.
A method of purifying a lentivirus particle comprising contacting the lentivirus particle with a protein-based purification matrix;
- wherein the lentivirus binds to the purification matrix to form a complex;
- wherein the size of the complex is increased by a first environmental factor;
- wherein the complex is separated from at least one contaminant on the basis of size;
- and
- wherein the biologic is separated from the purification matrix by a second environmental factor.
2 more elements
Dependencies are inferred from the claim text.