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AssigneeGammaDelta Therapeutics Ltd
InventorsShristi BHANDARI, Samuel FLORENCE, Andrew HUTTON, Louisa MATHIAS, Oliver NUSSBAUMER, Kalle SODERSTROM, Mark UDEN
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The present invention relates to a method for the isolation of lymphocytes (in particular γδ T cells) from a non-haematopoietic tissue sample comprising the steps of culturing a non-haematopoietic tissue sample which is an intact biopsy obtained from a non-haematopoietic tissue in the presence of Interleukin-2 (IL-2) and Interleukin-15 (IL-15); and collecting a population of lymphocytes cultured from the non-haematopoietic tissue sample. Methods of subsequent expansion are provided, as well as populations of isolated cells obtained by the method and uses thereof.
A method for the isolation of lymphocytes from a non-haematopoietic tissue sample comprising the steps of:
A method for the isolation of γδ T cells from a non-haematopoietic tissue sample comprising the steps of:
The method according to any preceding claim, wherein the non-haematopoietic tissue sample has a minimum cross-section of about 3 mm.
The method according to any preceding claim, wherein the non-haematopoietic tissue sample has a maximum cross-section of no greater than 8 mm.
The method according to any preceding claim, wherein the non-haematopoietic tissue sample has a maximum cross-section of no greater than 4 mm.
A method for the isolation of lymphocytes from a non-haematopoietic tissue sample comprising the steps of:
A method for the isolation of γδ T cells from a non-haematopoietic tissue sample comprising the steps of:
A method for the isolation of lymphocytes from a non-haematopoietic tissue sample comprising the steps of:
A method for the isolation of γδ T cells from a non-haematopoietic tissue sample comprising the steps of:
The method according to any preceding claim wherein the intact biopsy is obtained by punch biopsy.
A method for the isolation of lymphocytes from a non-haematopoietic tissue sample comprising the steps of:
A method for the isolation of γδ T cells from a non-haematopoietic tissue sample comprising the steps of:
The method according to any preceding claim, wherein the non-haematopoietic tissue sample is not minced prior to culturing.
The method according to any preceding claim, wherein the lymphocytes or γδ T cells are collected after at least 7 days of culturing.
The method according to any preceding claim, wherein the lymphocytes or γδ T cells are collected after at least 14 days of culturing.
The method according to any preceding claim, wherein the lymphocytes or γδ T cells are collected prior to 35 days of culturing.
The method according to any preceding claim, wherein the lymphocytes or γδ T cells are collected prior to 21 days of culturing.
The method according to any preceding claim, wherein the non-haematopoietic tissue sample is cultured in serum-free medium.
The method according any preceding claim, wherein the non-haematopoietic tissue sample is skin.
The method according any preceding claim, wherein the non-haematopoietic tissue sample is gut or gastrointestinal tract.
The method according to any preceding claim, wherein the non-haematopoietic tissue sample has been obtained from a human.
The method according to any preceding claim, wherein the IL-2 is human IL-2 or a functional equivalent thereof.
The method according to any preceding claim, wherein the IL15 is human IL-15 or a functional equivalent thereof.
The method according to any preceding claim, wherein the isolated population of cells comprise a population of Vδ1 T cells.
The method according to any preceding claim, further comprising expanding the isolated population of lymphocytes or γδ T cells.
A method for the isolation and expansion of lymphocytes from a non-haematopoietic tissue sample comprising the steps of:
A method for the isolation and expansion of γδ T cells from a non-haematopoietic tissue sample comprising the steps of:
Dependencies are inferred from the claim text.