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US 2022/0010252 A1Published application
Microphysiological choroid model
AssigneeFraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V.
InventorsChristopher Probst, Madalena Cipriano, Peter Loskill
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Abstract
The invention relates to the field of cultivating biological cells and tissues having an organ-like function on a microphysiological scale and provides a microphysiological reproduction of the choroid and the blood-retinal barrier as an in vitro test system.
Claims14
An in vitro tissue culture arrangement comprising:
- a first chamber (120) in a bioreactor (100),
- a 3D melanocyte culture (200) arranged in the first chamber (120), in which isolated melanocytes (220) are embedded in a hydrogel (240),
- a second chamber (140) in the bioreactor (100) which adjoins the first chamber (120) of the bioreactor (100),
- a first semipermeable membrane (130) which separates the second chamber (140) of the bioreactor (100) from the first chamber (120) of the bioreactor (100), wherein the membrane side (132) of the first semipermeable membrane (130) facing the first chamber (120) rests against the 3D melanocyte culture (200), and,
- a confluent first 2D endothelial cell layer (310) of isolated endothelial cells which is arranged in the second chamber (140) and rests against the membrane side (134) of the first semipermeable membrane (130) facing the second chamber (140).
2 more elements
Dependencies are inferred from the claim text.