Methods for inactivating and storing respiratory syncytial virus
AssigneesXiamen University, Xiamen Innovax Biotech Co., Ltd.
InventorsZizheng Zheng, Wei Zhang, Lujing Zhang, Yongpeng Sun, Li Chen, Ningshao Xia
Life of this patent
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Abstract
Provided are a method for inactivating respiratory syncytial virus (RSV) and stabilizing pre-F protein in RSV and inactivated RSV virus obtained thereby. Also provided are a vaccine comprising the inactivated RSV virus and a use of the vaccine in preventing or treating RSV infection or a disease related thereto.
Claims25
A method for inactivating an isolated respiratory syncytial virus (RSV) and stabilizing a pre-F protein in the RSV, which comprises the following steps:
- (1) providing an isolated live RSV;
- (2) fixing and inactivating the live RSV by using a fixing agent selected from the group consisting of:
- a formaldehyde solution having a formaldehyde concentration of about 0.015% to about 0.27% by weight (w/w, the same hereinafter) and a paraformaldehyde solution having a paraformaldehyde concentration of about 0.02% to about 0.3% by weight (w/w, the same hereinafter);
- (3) removing the fixing agent from the product of step (2), thereby obtaining an inactivated RSV.
2-3. (canceled)
A method for preserving a RSV and stabilizing a pre-F protein in the RSV, which comprises a step of placing the RSV in a storage solution, wherein the storage solution is a salt solution having an ion concentration of about 150 to about 1000 mM.
10-11. (canceled)
(canceled)
15-16. (canceled)
(canceled)
Dependencies are inferred from the claim text.