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US 2022/0009958 A1Published application
Methods of purification of albumin fusion proteins
AssigneeNovo Nordisk A/S
InventorsViveka Dolby, Are Bogsnes
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Abstract
The present invention provides a chromatographic separation method for improving the quality of albumin fusion protein solutions by removing impurities from the albumin fusion protein solution. This invention provides albumin fusion protein solution with a significantly reduced amount of the (yellow) coloured impurities and HCP.
Claims13
A method of purification of an aqueous albumin fusion protein solution, wherein said method comprises:
- (i) the step of loading said fusion protein solution onto a column comprising a cationic exchange chromatographic resin, and (ii) the step of eluting the fusion protein from the column using an elution buffer with a cation containing gradient.
Dependencies are inferred from the claim text.