Methods for preparing platelet releasate

A system for preparing a platelet releasate comprising a first container (1) having a first containing space (1′) wherein said first containing space (1′):

• is configured to receive a quantity (Qbc) of a platelet-rich blood composition comprised in a second containing space (2′) of a second container (2);
• holds a quantity (Qgp) of glass particles (3);
• and
• holds a quantity (Qcc) of one or more water-soluble calcium salts (4) or is configured to receive a quantity (Qcc) of one or more water-soluble calcium salts (4) held by a third containing space (5′) of a third container (5);
• wherein the ratio Qgp:Qbc is from 0.010 to 0.60 g per ml of said platelet-rich blood composition, and the ratio Qcc:Qbc is from 1.0 to 12.0 μmol per ml of said platelet-rich blood composition.

A method for preparing platelet releasate from a sample of a platelet-rich blood composition obtained from a subject comprising the steps of:

• a) contacting said sample with from 0.010 g to 0.60 g of glass particles per ml of said sample and from 1.0 μmol to 12.0 μmol of one or more water-soluble calcium salts per ml of said sample thereby obtaining a mixture;
• b) allowing the mixture obtained in step a) to coagulate, thereby obtaining a coagulum and platelet releasate;
• and
• c) recovering the platelet releasate obtained in step b) from the mixture.