Engineered regulatory t cells

A method of generating therapeutically effective numbers of eTregs for prophylactically and/or therapeutically treating a disease or condition in which it is desirable to suppress the immune system or reduce pro-inflammatory responses in a subject, said method comprising:

• providing a population of mononuclear cells in vitro;
• activating and expanding T cells from said mononuclear cells to yield a total T cell population;
• and
• transducing said total T cell population with a first nucleic acid construct encoding Ikaros transcription factor and with a second nucleic acid construct encoding FOXP3 to yield FOXP3+ and Ikaros transcription factor+ eTregs.