Modified t cell, preparation method therefor and use thereof
AssigneeInstitute of Zoology, Chinese Academy of Sciences
InventorsHaoyi WANG, Xingying ZHANG, Chen CHENG, Na TANG, Na LI
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Abstract
The present invention relates to the field of gene editing and tumor immunotherapy. In particular, the invention relates to methods for preparing modified T cells, such as CAR-T cells, by gene editing, and modified T cells prepared by the methods and uses thereof.
Claims24
A method for preparing a modified T cell, comprising a step of reducing or eliminating the expression of at least one inhibitory protein in the T cell, wherein the inhibitory protein is a T cell surface inhibitory receptor and/or a T cell exhaustion-related protein, for example, the inhibitory protein is selected from a TGFβ receptor (such as TGFBRII), TIGIT, BTLA, 2B4, CD160, CD200R, A2aR, IL10RA, ADRB2, BATF, GATA3, IRF4, RARA, LAYN, MYO7A, PHLDA1, RGS1, RGS2, SHP1, DGKa, Fas, FasL, or any combination thereof.
A modified T cell, wherein the expression of at least one inhibitory protein in the T cell is reduced or eliminated as compared with an unmodified T cell, wherein the inhibitory protein is a T cell surface inhibitory receptor and/or a T cell exhaustion-related protein, for example, the inhibitory protein is selected from a TGFβ receptor (such as TGFBRII), TIGIT, BTLA, 2B4, CD160, CD200R, A2aR, IL10RA, ADRB2, BATF, GATA3, IRF4, RARA, LAYN, MYO7A, PHLDA1, RGS1, RGS2, SHP1, DGKa, Fas, FasL, or any combination thereof.
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Dependencies are inferred from the claim text.