Microbial cells and methods for producing cannabinoids

A microbial cell for producing one or more cannabinoids, the microbial cell expressing a cannabinoid biosynthetic pathway comprising a heterologous prenyltransferase enzyme having cannabigerolic acid synthase (CBGAS) or cannabigerovarinic acid synthase (CBGVAS) activity,

• the microbial cell further comprising one or more modifications that increases carbon flux to geranyl diphosphate (GPP) and/or carbon flux to one or more of hexanoic acid, hexanoyl-CoA, butyric acid, butyryl-CoA, and/or acetyl-CoA;
• and/or
• the microbial cell produces the cannabinoid from one or more fed precursors selected from olivetol, olivetolic acid, divarin, divarinic acid, hexanoic acid, butyric acid, hexanoyl-CoA, butyryl-CoA, or derivative thereof and/or GPP precursor.