Use of oligodendrocytes from oral neuroectodermal stem cells in the repair of the nervous system
AssigneesAssistance Publique-Hopitaux de Paris, Université Paris 5 René Descartes, Universite Paris Diderot—Paris 7, Universite Paris Est Creteil Val De Marne
InventorsBenjamin FOURNIER, Bruno GOGLY, Catherine MARCHAND-LEROUX, Ali NASSIF, Ihsène TAÏHI
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Abstract
This invention concerns a new method for differentiating oral neuroectodermal stem cells (CSO-NE), in particular human gingival neuroectodermal stem cells (CSGh), into oligodendrocytes (OL), and their use in the repair of the nervous system, in particular of head injuries.
Claims10
A method for inducing differentiation of oral neuroectodermal stem cells into oligodendrocytes, said method comprising:
- a) a step of differentiating said neuroectodermal oral stem cells into neuroepithelial stem cells in a neurogenic culture medium comprising DMEMf12, N 1 supplement, FGFb and EGF.
- b) a step of differentiating the neuroepithelial stern cells obtained in step a) into neural stem cells in a neurogenic culture medium comprising DMEMf12, N1 supplement, Noggin protein, FGFb, retinoic acid and SHH factor.
- c) a step of differentiating the neural stem cells obtained in step b) into oligodendrocyte pre-precursor cells in a neurogenic culture medium comprising DMEMf12, N1 supplement, B27 supplement and FGFb,
- d) a step of differentiating the oligodendrocyte pre-precursor cells obtained in step c) into oligodendrocyte precursor cells in a neurogenic culture medium comprising
- DMEMf12, PDGF-AA and SHH factor.
- e) a step of differentiating oligodendrocyte precursor cells obtained in step d) into oligodendrocytes, and maturing said oligodendrocytes, in a neurogenic culture medium comprising DMEMf12, ICE-1 , biotin, CAMP, PDGF-AA, L-glutamine or Cilutamax™ and NT3.
Culture medium for the differentiation of oral neuroectodermal stem cells into neuroepithelial stem cells comprising DMEMf12, N 1 supplement, FGFb and EGF.
Culture medium for the differentiation of neuroepithelial stem cells into neural stem cells comprising DMFMf12, N1 supplement, Noggin protein, FGFb, retinoic acid and factor.
Culture medium for the differentiation of neural stem cells into oligodendrocyte pre-precursor cells comprising DMFMf12, N1 supplement, B27 supplement and FGFb.
Culture medium for the differentiation of oligodendrocyte pre-precursor cells into oligodendrocyte precursor cells comprising DMEMf12, PDGF-AA and SHH factor.
Culture medium for the differentiation of oligodendrocyte precursor cells into oligodendrocytes, and for the maturation of said oligodendrocytes, comprising DMEMf12, IGF-1 biotin, CAMP, PDGF-AA, L-glutamine or Glutamax™ and NT3.
Dependencies are inferred from the claim text.