Alpha-amylase variants having improved performance and stability
AssigneeNovozymes A/S
InventorCarsten Andersen
Life of this patent
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Abstract
The present invention relates to alpha-amylase variants. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.
Claims27
An alpha-amylase variant of a parent alpha-amylase, wherein said variant is a mature form of an alpha-amylase having amylase activity and wherein said variant has an improved stability and performance, wherein said variant has an Improvement Factor (IF) of >1.0 for a measure of stability and an IF of >1.0 for a measure of performance and wherein said variant has at least 65% but less than 100% sequence identity to said parent alpha-amylase.
A method for obtaining an alpha-amylase variant, comprising introducing into a parent alpha-amylase a modification at one or more positions wherein said one or more modifications provides a variant having an Improvement Factor (IF) of >1.0 for a measure of stability and an IF of >1.0 for a measure of performance and having alpha-amylase activity;
- and recovering said variant.
A method of improving the stability and performance of a parent alpha-amylase having the amino acid sequence set forth in SEQ ID NOs:
- 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, or 13, or having at least 65% sequence identity to any one of the amino acid sequences set forth in SEQ ID NOs:
- 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, or 13, said method optionally comprises introducing one or more modifications to said parent alpha-amylase;
- wherein said resulting variant has at least 65% but less than 100% sequence identity to the amino acid sequence as set forth in SEQ ID NOs:
- 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, or 13, and wherein said variant has alpha-amylase activity, improved stability and improved performance as compared to said parent alpha-amylase.
Dependencies are inferred from the claim text.