Back to results
US 2021/0343515 A1Published application
Systems and methods for microarray droplet ionization analysis
AssigneeBoard of Regents, The University of Texas System
InventorsLivia Schiavinato EBERLIN, Marta Sans ESCOFET, Bryan R. WYGANT, Jialing ZHANG, C. Buddie MULLINS
Life of this patent
Estimating the term…
Abstract
Method and devices are provided for imaging a surface, such as a biological tissue sample, by mass spectrometry. In certain aspects, devices of the embodiments allow for the placement and collection of a plurality of spatially separated liquid droplets on a sample and delivery of the droplets with extracted sample analytes for mass spectrometry analysis.
Claims67
An apparatus for producing samples for mass spectrometry analysis, the apparatus comprising:
- a solvent dispenser configured to dispense single droplets of solvent on a sample comprising an analyte;
- an electrically conductive conduit configured to transfer the droplets of solvent and the analyte from the sample to a mass spectrometer and that allow a voltage potential to be applied to the droplets;
- and, optionally,
- a heated conduit configured to increase the temperature of the droplets of solvent and the analyte.
1 more element
A method for imaging a surface comprising:
- (a) applying a discrete volume of a solvent to a plurality of distinct sites on the surface, the discrete volume of solvent being applied through a dispenser;
- (b) individually collecting and ionizing the discrete volumes of applied solvent to obtain a plurality of ionized liquid samples, wherein the collecting is through a solvent conduit;
- and
- (c) individually subjecting the plurality of ionized liquid samples to mass spectrometry analysis.
1 more element
Dependencies are inferred from the claim text.