Method and composition for determining specific antibody responses to species of filovirus
AssigneeThe Government of the United States, as represented by the Secretary of the Army
InventorsRobert G. ULRICH, Teddy KAMATA
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Abstract
The disclosure relates to compositions, assays, methods and kits comprising one or more amino acid sequences of a filovirus protein, or a fragment thereof, which find use in the detection of a filovirus infection and/or the presence of antibodies specific for a filovirus in a biological sample.
Claims49
A detection agent comprising one or more amino acid sequences of a filovirus protein, or a fragment thereof, and a substrate, wherein the one or more amino acid sequences of the filovirus protein is attached to the substrate.
A method for detecting the presence of filovirus-specific antibody in biological sample obtained from a subject comprising:
- (a) incubating the biological sample with a detection agent comprising one or more amino acid sequences of a filovirus protein, or a fragment thereof, attached to a substrate under conditions that allow binding of the filovirus-specific antibody to the detection agent;
- and
- (b) detecting the filovirus-specific antibody bound to detection agent.
A method for identifying a subject infected with a filovirus, comprising:
- determining whether a filovirus-specific antibody is present in a sample obtained from the subject, wherein the determining comprises:
- (a) incubating the biological sample with a detection agent comprising one or more amino acid sequences of a filovirus protein, or a fragment thereof, attached to a substrate under conditions that allow binding of the filovirus-specific antibody to the detection agent;
- and
- (b) detecting the filovirus-specific antibody bound to the detection agent,
- wherein the detection of the filovirus-specific antibody identifies that the subject is infected with a filovirus.
A method for identifying whether a subject is infected with a filovirus, comprising:
- determining whether an antibody to the filovirus is present in a sample obtained from the subject, wherein the determining comprises:
- (a) contacting the sample with at least one protein, or a fragment thereof, from the filovirus to which the antibody can specifically bind;
- and
- (b) detecting specific binding between the at least one protein and the antibody, wherein the detection of specific binding identifies that the subject is infected with a filovirus.
Dependencies are inferred from the claim text.