Biosensor using magnetic nanoparticles, and detection device and detection method using same

A biosensor for detecting a target antigen, the biosensor comprising:

• a sample introduction part;
• a reaction part comprising a first electrode, wherein a first antibody is positioned on the first electrode, the first electrode is configured to electrically connect with an electrode of a detection device;
• and
• a magnetic nanoparticle complex comprising a first antigen, a reaction substance and a magnetic nanoparticle, wherein the magnetic nanoparticle has a magnetic property, and the first antigen and the reaction substance are positioned on the magnetic nanoparticle;
• wherein the sample introduction part is configured to make fluidic connect with the reaction part such that a sample introduced through the sample introduction part moves to the reaction part;
• wherein the first antibody positioned on the first electrode specifically binds to the target antigen,
• wherein the first antigen of the magnetic nanoparticle complex specifically binds to the first antibody, and
• wherein the reaction substance has a property that induces a change of a current according to a voltage applied between the first electrode and a second electrode when the reaction substance is near the first electrode, to represent the target antigen is included in the sample.

A biosensor for detecting a target antibody, the biosensor comprising:

• a sample introduction part;
• a reaction part comprising a first electrode, wherein a first antigen is positioned on the first electrode, the first electrode is configured to electrically connect with an electrode of a detection device;
• and
• a magnetic nanoparticle complex comprising a second antibody, a reaction substance and a magnetic nanoparticle, wherein the magnetic nanoparticle has a magnetic property, and the second antibody and the reaction substance are positioned on the magnetic nanoparticle;
• wherein the sample introduction part is configured to make fluidic connect with the reaction part such that a sample introduced through the sample introduction part moves to the reaction part;
• wherein the first antigen positioned on the first electrode specifically binds to the target antibody,
• wherein the second antibody of the magnetic nanoparticle complex specifically binds to the first antigen, and
• wherein the reaction substance has a property that induces a change of a current according to a voltage applied between the first electrode and a second electrode when the reaction substance is near the first electrode, to represent the target antibody is included in the sample.

A biosensor for detecting a target antibody, the biosensor comprising:

• a sample introduction part;
• a reaction part comprising a first electrode, wherein a first antigen is positioned on the first electrode, the first electrode is configured to electrically connect with an electrode of a detection device;
• and
• a magnetic nanoparticle complex comprising a second antigen, a reaction substance and a magnetic nanoparticle, wherein the magnetic nanoparticle has a magnetic property, and the second antigen and the reaction substance are positioned on the magnetic nanoparticle;
• wherein the sample introduction part is configured to make fluidic connect with the reaction part such that a sample introduced through the sample introduction part moves to the reaction part;
• wherein the first antigen positioned on the first electrode specifically binds to the target antibody,
• wherein the second antigen of the magnetic nanoparticle complex specifically binds to the target antibody, and
• wherein the reaction substance has a property that induces a change of a current according to a voltage applied between the first electrode and a second electrode when the reaction substance is near the first electrode, to represent the target antibody is included in the sample.