Method of quantitative measurement of particle content using hydrated state imaging
AssigneeIntelligent Virus Imaging Inc.
InventorsMathieu Colomb-Delsuc, Lars Haag, Rickard Nordström, Martin Ryner
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Abstract
The method is for quantitative measurement of particle content using hydrated state imaging such as CryoTEM. A sample of virus-like particles (VLPs) or virus particles is provided. Preferably, the sample is rapidly frozen into a cryogenic liquid at a cryogenic temperature. While at the cryogenic temperature, the particle content of each VLP in the frozen sample is observed in the CryoTEM. An amount of the particle content of the VLPs is determined to assess whether the VLPs are empty or not.
Claims11
A method for assessing a particle interior content using imaging, comprising:
- providing a sample of virus-like particles (VLPs) or virus particles;
- maintaining the sample in a native unstained hydrated state;
- imaging the sample in the native unstained hydrated state in an Electron Microscope;
- observing the particle interior content of each virus-like particle (VLP) or virus AAV particle in the sample in acquired images in the Electron Microscope;
- and
- based on the observation of the particle interior content, measuring an intensity of the particle interior content of each virus-like particle (VLP) or virus particle in the sample;
- based on the measured intensity, determining an amount of the particle interior content of each virus-like particle (VLP) or virus particle in the sample and assessing whether the particles are empty, full or ambiguous.
Dependencies are inferred from the claim text.