Compositions for detecting candida species

A reaction mixture for determining the presence or absence of Candida species (sp.) in a sample, wherein the Candida sp. is one or more of C. albicans, C. parapsilosis, C. dubliniensis, C. tropicalis, and C. glabrata, the reaction mixture comprising:

• a first amplification oligomer combination and a second amplification oligomer combination, wherein
• (a) the first amplification oligomer combination comprises first and second Candida-specific amplification oligomers for amplifying a first Candida sp. nucleic acid target region or a second Candida sp. nucleic acid target region, wherein said first target region corresponds to a region of SEQ ID NO:129 from about nucleotide position 133 or 161 to about nucleotide position 259 and said second region corresponds to a region of SEQ ID NO:130 from about nucleotide position 202 to about nucleotide position 308, and wherein the first and second Candida-specific amplification oligomers respectively comprise first and second Candida-specific target-hybridizing sequences, wherein
• the first Candida-specific target-hybridizing sequence consists of the nucleotide sequence of residues 28-46 of SEQ ID NO:9;
• and
• the second Candida-specific target-hybridizing sequence consists of the nucleotide sequence of SEQ ID NO:26;
• and
• (b) the second amplification oligomer combination comprises first and second C. glabrata-specific amplification oligomers for amplifying a third Candida sp. nucleic acid target region, wherein said third target region corresponds to a region of SEQ ID NO:131 from about nucleotide position 355 to about nucleotide position 554, and wherein the first and second C. glabrata-specific amplification oligomers respectively comprise first and second C. glabrata-specific target-hybridizing sequences, wherein
• the first C. glabrata-specific target-hybridizing sequence is a sequence of from 15 to 24 contiguous nucleotides contained in the sequence of SEQ ID NO:134 and that includes at least the sequence of SEQ ID NO:135;
• and/or
• the second C. glabrata-specific target-hybridizing sequence is a sequence of from 16 to 21 contiguous nucleotides contained in the sequence of SEQ ID NO:136 and that includes at least the sequence of SEQ ID NO:137;
• wherein each of the first Candida-specific amplification oligomer and the first C. glabrata-specific amplification oligomer is a promoter primer or promoter provider further comprising a promoter sequence located 5′ to the respective target-hybridizing sequence, and
• wherein each of the second Candida-specific amplification oligomer and the second C. glabrata-specific amplification oligomer is a non-promoter primer;
• and
• a Candida-specific detection probe that specifically hybridizes to the first or second Candida sp. target region and a C. glabrata-specific detection probe that specifically hybridizes to the third Candida sp. target region, wherein each of the Candida-specific and C. glabrata-specific detection probes is a molecular torch.