Blood Sample Processing and Nucleic Acid Amplification Systems, Devices, and Methods
AssigneeThe Trustees of Columbia University in the City of New York
InventorsSamuel K. SIA, Nicole R. BLUMENFELD, Rodrigo C. CHAVES, Giuseppe MODICA, Michael LUETOLF
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Abstract
Aspects described herein provide blood sample purification and microfluidic nucleic acid amplification systems and methods for purifying blood samples and amplifying a target nucleic acid on the same system. The described systems and methods include a filter component and a microfluidic amplification component for amplification of nucleic acid from the blood sample, and can be used, for example, for point of care blood analysis.
Claims20
A blood sample preparation system comprising:
- a filter component comprising at least a first filter and a second filter for preparing a blood sample, each filter having a different pore size;
- and
- a microfluidic amplification component for amplification of nucleic acid from the blood sample.
A method of filtering and amplifying a nucleic acid target from a blood sample, comprising:
- providing a blood sample to a filtering component;
- filtering the blood sample through a first filter and a second filter to form a filtered blood sample;
- and
- amplifying the nucleic acid target from the filtered blood sample.
A method of filtering a blood sample and amplifying a nucleic acid target from the blood sample, comprising:
- providing a blood sample to a filtering component;
- filtering the blood sample through a first filter and a second filter to form a filtered blood sample;
- purifying nucleic acid from the filtered blood sample to form a purified nucleic acid sample;
- and
- amplifying the nucleic acid target from the purified nucleic sample.
Dependencies are inferred from the claim text.