Identification of dna polymerase theta inactivation mechanism
AssigneeThe Johns Hopkins University
InventorsMarc Greenberg, Daniel Laverty
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Abstract
Methods for identifying one or more Pol θ inhibitors and methods of use thereof are disclosed.
Claims25
A method for identifying a DNA polymerase theta (Pol θ) inhibitor, the method comprising:
- (a) contacting Pol θ with one or more candidate Pol θ inhibitors in a Pol θ reaction buffer to form a pre-incubation mixture;
- (b) incubating the pre-incubation mixture for a period of time at room temperature;
- (c) diluting the pre-incubation mixture with a solution containing a DNA substrate and nucleotide triphosphates in a Pol θ reaction buffer;
- and
- (d) measuring fluorescence of the diluted pre-incubation mixture for a period of time.
A method for inhibiting Pol θ activity in a subject, the method comprising administering to the subject a therapeutically effective amount of a Pol θ inhibitor, or an analogue or a pharmaceutically acceptable salt thereof.
A method for inhibiting a cancer cell, the method comprising contacting the cancer or a noncancerous cell with a Pol θ inhibitor in an amount effective to inhibit Pol θ.
A method for treating a cancer in a subject, the method comprising administering to the subject in need of treatment thereof a therapeutically effective amount of a Pol θ inhibitor, or an analogue or a pharmaceutically acceptable salt thereof.
Dependencies are inferred from the claim text.