Manganese supplementation for control of glycosylation in mammalian cell culture process
AssigneeBiogen MA Inc.
InventorsLia Tescione, Thomas Ryll, Alan Gilbert
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Abstract
The present invention pertains to a cell culture medium comprising manganese as a media supplement, which was shown to control recombinant protein glycosylation and methods of using thereof. The present invention further pertains to a method of controlling or manipulating glycosylation of a recombinant protein of interest in a large scale cell culture.
Claims28
A method for achieving a predetermined glycosylation profile of an anti-α4-integrin antibody comprising providing manganese to a cell culture at a concentration that falls within a target manganese concentration range, wherein the cell culture comprises host cells producing the anti-α4-integrin antibody.
A method for achieving a predetermined glycosylation profile of an anti-α4-integrin antibody comprising (i) determining a manganese concentration in a component of a cell culture medium, (ii) if the manganese concentration is below a target manganese concentration range, supplementing the cell culture medium with the component to achieve a manganese concentration within the target manganese concentration range, and (iii) culturing a recombinant host cell producing an anti-α4-integrin antibody in the cell culture medium comprising the cell culture medium component.
A method for achieving a predetermined glycosylation profile of an anti-α4-integrin antibody comprising (i) determining a manganese concentration in a component of a cell culture medium, (ii) if the manganese concentration is below a target manganese concentration range, adding manganese to the component of the cell culture medium to achieve a manganese concentration within the target manganese concentration range, (iii) producing a cell culture medium using the component of cell culture medium with the target manganese concentration, and (iv) culturing a recombinant host cell producing an anti-α4-integrin antibody in the cell culture medium comprising the cell culture medium component with the target manganese concentration.
A method for optimizing a cell culture medium for the production of an anti-α4-integrin antibody comprising (i) determining the amount of manganese in a cell culture medium or a component used to produce a cell culture medium, and (ii) if the amount of manganese is below a target range, supplementing the cell culture medium or the component of the cell culture medium with manganese to achieve an amount of manganese within the target range, wherein the target range is sufficient to produce anti-α4-integrin antibody with a predetermined glycosylation profile.
A method for optimizing a cell culture medium for the production of an anti-α4-integrin antibody comprising (i) determining the amount of manganese in a cell culture medium or a component used to produce a cell culture medium, and (ii) if the amount of manganese is above a target range, removing manganese from the cell culture medium or the component of the cell culture medium to achieve an amount of manganese within the target range, wherein the target range is sufficient to produce anti-α4-integrin antibody with a predetermined glycosylation profile.
8-9. (canceled)
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A method for achieving a predetermined glycosylation profile of an interferon beta-1a polypeptide comprising providing manganese to a cell culture at a concentration that falls within a target manganese concentration range, wherein the cell culture comprises host cells producing the interferon beta-1a polypeptide.
A method for achieving a predetermined glycosylation profile of an interferon beta-1a polypeptide comprising (i) determining a manganese concentration in a component of a cell culture medium, (ii) if the manganese concentration is below a target manganese concentration range, supplementing the cell culture medium with the component to achieve a manganese concentration within the target manganese concentration range, and (iii) culturing a recombinant host cell producing an interferon beta-1a polypeptide in the cell culture medium comprising the cell culture medium component.
A method for achieving a predetermined glycosylation profile of an interferon beta-1a polypeptide comprising (i) determining a manganese concentration in a component of a cell culture medium, (ii) if the manganese concentration is below a target manganese concentration range, adding manganese to the component of the cell culture medium to achieve a manganese concentration within the target manganese concentration range, (iii) producing a cell culture medium using the component of cell culture medium with the target manganese concentration, and (iv) culturing a recombinant host cell producing an interferon beta-1a polypeptide in the cell culture medium comprising the cell culture medium component with the target manganese concentration.
A method for optimizing a cell culture medium for the production of an interferon beta-1a polypeptide comprising (i) determining the amount of manganese in a cell culture medium or a component used to produce a cell culture medium, and (ii) if the amount of manganese is below a target range, supplementing the cell culture medium or the component of the cell culture medium with manganese to achieve an amount of manganese within the target range, wherein the target range is sufficient to produce interferon beta-1a polypeptide with a predetermined glycosylation profile.
A method for optimizing a cell culture medium for the production of an interferon beta-1a polypeptide comprising (i) determining the amount of manganese in a cell culture medium or a component used to produce a cell culture medium, and (ii) if the amount of manganese is above a target range, removing manganese from the cell culture medium or the component of the cell culture medium to achieve an amount of manganese within the target range, wherein the target range is sufficient to produce interferon beta-1a polypeptide with a predetermined glycosylation profile.
32-33. (canceled)
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50-77. (canceled)
Dependencies are inferred from the claim text.