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US 2021/0340576 A1Published application
Method for the monitoring of modified nucleases induced-gene editing events by molecular combing
AssigneeGenomic Vision
InventorsSebastien BARRADEAU, Aaron BENSIMON, Laurent CAVAREC
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Abstract
Methods for detecting and characterizing large genomic rearrangements induced by modified nucleases at high resolution and for quantifying the frequency of the large genomic or gene rearrangements induced by modified nucleases using Molecular Combing.
Claims21
A method for detecting, characterizing, quantifying, or
- determining the efficiency of, a gene or genome editing procedure or event comprising:
- editing a target nucleic acid(s) in a gene or genome and detecting or quantifying at least one genetic modification, deletion, duplication, amplification, translocation, insertion or inversion in the edited target nucleic acid using molecular combing.
A method for determining the efficiency, accuracy or specificity of a polynucleotide editing procedure that uses at least one modified nuclease comprising:
- (i) editing one or more polynucleotide(s) of interest using at least one modified nuclease,
- (ii) contacting the edited polynucleotide(s) with labelled polynucleotide(s) that hybridize to them and performing molecular combing of the fluorescent labeled polynucleotides, and
- (iii) comparing the edited polynucleotides hybridized to said labelled polynucleotides to one or more control polynucleotides, which have not been treated with the modified nuclease, hybridized to said labelled polynucleotide(s), thus determining the efficiency, accuracy or specificity of the polynucleotide editing procedure using the modified nuclease;
- and
- (iv) optionally, selecting a modified nuclease based polynucleotide editing procedure that is most accurate or efficient for correction or modification of a particular polynucleotide of interest.
2 more elements
Dependencies are inferred from the claim text.