High-content imaging of microfluidic devices
AssigneeEmulate, Inc.
InventorsKyung-Jin Jang, Daniel Levner, Konstantia-Roumvini Kodella, Jonathan Rubins, Debora Barreiros Petropolis, matt Boeckeler, Geraldine Hamilton
Life of this patent
Estimating the term…
Abstract
The present invention is related to high-content microscopy imaging of microfluidic cell culture systems. A method of high-content microfluidic device microscopy is contemplated, along with related statistical analysis and microfluidic device adaptors.
Claims51
1-110. (canceled)
A method of applying lipid nanoparticles (LNPs) to cells, comprising a) providing i) a plurality of lipid nanoparticles (LNPs) ii) a microfluidic device comprising a first microchannel seeded with cells of a first cell type, and b) introducing said LNPs into said microfluidic device.
A method of applying lipid nanoparticles (LNP) to cells in a microfluidic device, comprising a) providing i) a plurality of lipid nanoparticles (LNP) comprising nucleic acid sequences;
- ii) a microfluidic device comprising a microchannel seeded with cells of a first cell type, and b) flowing said LNPs into said microfluidic device for delivering said nucleic acid sequences to said cells.
A method of applying Adeno-Associated Virus (AAV) vectors to cells, comprising a) providing i) a plurality of Adeno-Associated Virus (AAV) vectors ii) a microfluidic device comprising a first microchannel seeded with cells of a first cell type, and b) introducing said AAV vectors into said microfluidic device.
A method of delivering nucleic acid sequences to cells in a microfluidic device, comprising a) providing i) a plurality of Adeno-Associated Virus (AAV) vectors comprising nucleic acid sequences;
- ii) a microfluidic device comprising a microfluidic channel seeded with cells of a first cell type, and b) flowing said AAV vectors into said microchannel for delivering said sequences to said cells.
Dependencies are inferred from the claim text.