Compositions and methods for increasing the efficiency of cell cultures used for food production
AssigneeUpside Foods, Inc.
InventorsNicholas J. GENOVESE, Eric N. SCHULZE, Danielle N. DESMET
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Abstract
Provided herein are compositions and methods to make and use engineered cells, for the purpose of increasing the cell density of a culture comprising metazoan cells and for the production of a cultured edible product.
Claims27
-2. (canceled)
A method for increasing the cell density of a culture comprising metazoan cells, the method comprising:
- a. introducing into the cells one or more polynucleotide sequences encoding glutamine synthetase (GS), insulin-like growth factor (IGF), albumin or a combination thereof;
- b. introducing into the cells a polynucleotide sequence encoding a telomerase reverse transcriptase (TERT);
- and
- c. culturing the cells in a cultivation infrastructure.
The method of claim 1, wherein the cells comprise a loss-of-function mutation in one or more genes encoding cyclin-dependent kinase inhibitor (CKI) proteins.
(canceled)
The method of claim 1, wherein the cells are from a self-renewing cell line.
(canceled)
The method of claim 1, wherein the cells are modified with a myogenic transcription factor.
(canceled)
The method of claim 1, wherein:
- a. the concentration of glutamine in the culture medium is increased to at least 0.001 mM;
- b. the concentration of IGF in the culture medium is increased to at least 0.001 ng/mL;
- and/or
- c. the concentration of albumin in the culture medium is increased to at least 0.0001 mg/mL,
- compared to cultures of cells in which the expression of GS, IGF, albumin or a combination thereof is not increased.
The method of claim 1, comprising inhibiting the HIPPO signaling pathway.
The method of claim 1, wherein the cells are from a livestock, poultry, game or aquatic animal species.
The method of claim 1, wherein the cells are from a chicken, duck, or turkey.
The method of claim 1, wherein the cells are from a fish.
The method of claim 1, wherein the cells are from a livestock species.
The method of claim 1, wherein the cells are from any animal species intended for human or non-human dietary consumption.
The method of claim 1, wherein the cells are myogenic cells.
(canceled)
The method of claim 1, wherein the cells are non-myogenic cells.
The method of claim 1, wherein the cells are non-myogenic cells modified to express one or more myogenic transcription factors.
(canceled)
The method of claim 1, wherein the polynucleotide sequence encoding GS comprises a GS coding sequence from Tables 1A and 1B, wherein the polynucleotide sequence encoding IGF comprises an IGF coding sequence from Tables 1A and 1B, or wherein the polynucleotide sequence encoding albumin comprises an albumin coding sequence from Tables 1A and 1B.
-49. (canceled)
A method of decreasing the concentration of ammonia and/or ammonium hydroxide in the medium of cells in culture comprising increasing the expression of glutamine synthetase (GS) protein in the cells, wherein the cells are of livestock, poultry, game or aquatic animal species, and wherein the concentration of ammonia (i.e. ammonium hydroxide) in the medium is decreased by at least 2.5%.
-127. (canceled)
Dependencies are inferred from the claim text.