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AssigneeBoard of Regents, The University of Texas System
InventorShawn Christensen
Estimating the term…
Engineered transposons and methods of use thereof are provided. The transposons typically include an RNA component and a protein component. The RNA component can include, for example, a DNA targeting sequence, one or more protein binding motifs, and a nucleic acid sequence of interest to be integrated into a target DNA. The protein component is typically derived from a RLE LINE element protein and can include a DNA binding domain, an RNA binding domain, a reverse transcriptase, a linker domain, and an endonuclease. Pharmaceutical compositions and methods of use for introducing nucleic acid sequences into the genomes of cells are also provided.
A RNA component comprising a DNA targeting sequence, one or more protein binding motifs (PBM), and a nucleic acid sequence of interest to be integrated into a DNA target site, wherein the DNA targeting sequence, the protein binding motifs, and sequence of interest are operably linked such that they can bind to a protein component derived from a parental Long Interspersed (LINE) element protein and be reverse transcribed into cDNA and the cDNA can be integrated into the DNA at the DNA target site.
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A protein component comprising a DNA binding domain, an RNA binding domain, a reverse transcriptase, a linker domain, and an endonuclease wherein the DNA binding domain, RNA binding domain, reverse transcriptase, linker domain, and endonuclease are operably linked such that they can bind to an RNA component and DNA at a DNA target site, and facilitate reverse transcription of the RNA component into cDNA, and integration of the cDNA into the DNA at the DNA target site.
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Dependencies are inferred from the claim text.