US 2021/0340556 A1 · TRANSGENIC PLANT COMPRISING A POLYNUCLEOTIDE ENCODING A VARIABLE DOMAIN OF HEAVY-CHAIN ANTIBODY

Abstract

This disclosure relates to a transgenic plant or plant tissue. In particular, this disclosure relates to a transgenic plant or plant tissue or plant cell comprising at least one polynucleotide comprising at least one sequence encoding a variable domain of a heavy-chain antibody (VHH) specifically binding to a sphingolipid of a fungus. Advantageously, the expression of the polynucleotide in at least part of the transgenic plant or plant tissue or plant cell (i) protects at least part of the transgenic plant or plant tissue or plant cell from an infection with a plant pathogenic fungus, (ii) inhibits the growth of a plant pathogenic fungus on at least part of the transgenic plant or plant tissue or plant cell, or (iii) increases the resistance of at least part of the transgenic plant or plant tissue or plant cell against a plant pathogenic fungus. This disclosure also relates to a method for protecting at least part of a plant or plant tissue or plant cell from an infection with a plant pathogen, for inhibiting the growth of a plant pathogen on at least part of a plant or plant tissue or plant cell, or for increasing pathogen resistance of at least part of a plant or plant tissue or plant cell, comprising expressing in at least part of the plant or plant tissue or plant cell at least one polynucleotide encoding a VHH specifically binding to a pathogen.

Claims26

Dependent claims

1-18. (canceled)

A transgenic plant or plant tissue or plant cell comprising at least one polynucleotide comprising at least one sequence encoding a variable domain of a heavy-chain antibody (VHH) that specifically binds to a glucosylceramide of a fungus;

wherein the at least one polynucleotide comprises a sequence encoding a VHH comprising:
(i) any one or more of SEQ ID NOS:1 to 84, and/or
(ii) a CDR1 region having SEQ ID NO:
85, a CDR2 region having SEQ ID NO:
169, and a CDR3 region having SEQ ID NO:
253, or
a CDR1 region having SEQ ID NO:
86, a CDR2 region having SEQ ID NO:
170, and a CDR3 region having SEQ ID NO:
254, or
a CDR1 region having SEQ ID NO:
87, a CDR2 region having SEQ ID NO:
171, and a CDR3 region having SEQ ID NO:
255, or
a CDR1 region having SEQ ID NO:
88, a CDR2 region having SEQ ID NO:
172, and a CDR3 region having SEQ ID NO:
256, or
a CDR1 region having SEQ ID NO:
89, a CDR2 region having SEQ ID NO:
173, and a CDR3 region having SEQ ID NO:
257, or
a CDR1 region having SEQ ID NO:
90, a CDR2 region having SEQ ID NO:
174, and a CDR3 region having SEQ ID NO:
258, or
a CDR1 region having SEQ ID NO:
91, a CDR2 region having SEQ ID NO:
175, and a CDR3 region having SEQ ID NO:
259, or
a CDR1 region having SEQ ID NO:
92, a CDR2 region having SEQ ID NO:
176, and a CDR3 region having SEQ ID NO:
260, or
a CDR1 region having SEQ ID NO:
93, a CDR2 region having SEQ ID NO:
177, and a CDR3 region having SEQ ID NO:
261, or
a CDR1 region having SEQ ID NO:
94, a CDR2 region having SEQ ID NO:
178, and a CDR3 region having SEQ ID NO:
262, or
a CDR1 region having SEQ ID NO:
95, a CDR2 region having SEQ ID NO:
179, and a CDR3 region having SEQ ID NO:
263, or
a CDR1 region having SEQ ID NO:
96, a CDR2 region having SEQ ID NO:
180, and a CDR3 region having SEQ ID NO:
264, or
a CDR1 region having SEQ ID NO:
97, a CDR2 region having SEQ ID NO:
181, and a CDR3 region having SEQ ID NO:
265, or
a CDR1 region having SEQ ID NO:
98, a CDR2 region having SEQ ID NO:
182, and a CDR3 region having SEQ ID NO:
266, or
a CDR1 region having SEQ ID NO:
99, a CDR2 region having SEQ ID NO:
183, and a CDR3 region having SEQ ID NO:
267, or
a CDR1 region having SEQ ID NO:
100, a CDR2 region having SEQ ID NO:
184, and a CDR3 region having SEQ ID NO:
268, or
a CDR1 region having SEQ ID NO:
101, a CDR2 region having SEQ ID NO:
185, and a CDR3 region having SEQ ID NO:
269, or
a CDR1 region having SEQ ID NO:
102, a CDR2 region having SEQ ID NO:
186, and a CDR3 region having SEQ ID NO:
270, or
a CDR1 region having SEQ ID NO:
103, a CDR2 region having SEQ ID NO:
187, and a CDR3 region having SEQ ID NO:
271, or
a CDR1 region having SEQ ID NO:
104, a CDR2 region having SEQ ID NO:
188, and a CDR3 region having SEQ ID NO:
272, or
a CDR1 region having SEQ ID NO:
105, a CDR2 region having SEQ ID NO:
189, and a CDR3 region having SEQ ID NO:
273, or
a CDR1 region having SEQ ID NO:
106, a CDR2 region having SEQ ID NO:
190, and a CDR3 region having SEQ ID NO:
274, or
a CDR1 region having SEQ ID NO:
107, a CDR2 region having SEQ ID NO:
191, and a CDR3 region having SEQ ID NO:
275, or
a CDR1 region having SEQ ID NO:
108, a CDR2 region having SEQ ID NO:
192, and a CDR3 region having SEQ ID NO:
276, or
a CDR1 region having SEQ ID NO:
109, a CDR2 region having SEQ ID NO:
193, and a CDR3 region having SEQ ID NO:
277, or
a CDR1 region having SEQ ID NO:
110, a CDR2 region having SEQ ID NO:
194, and a CDR3 region having SEQ ID NO:
278, or
a CDR1 region having SEQ ID NO:
111, a CDR2 region having SEQ ID NO:
195, and a CDR3 region having SEQ ID NO:
279, or
a CDR1 region having SEQ ID NO:
112, a CDR2 region having SEQ ID NO:
196, and a CDR3 region having SEQ ID NO:
280, or
a CDR1 region having SEQ ID NO:
113, a CDR2 region having SEQ ID NO:
197, and a CDR3 region having SEQ ID NO:
281, or
a CDR1 region having SEQ ID NO:
114, a CDR2 region having SEQ ID NO:
198, and a CDR3 region having SEQ ID NO:
282, or
a CDR1 region having SEQ ID NO:
115, a CDR2 region having SEQ ID NO:
199, and a CDR3 region having SEQ ID NO:
283, or
a CDR1 region having SEQ ID NO:
116, a CDR2 region having SEQ ID NO:
200, and a CDR3 region having SEQ ID NO:
284, or
a CDR1 region having SEQ ID NO:
117, a CDR2 region having SEQ ID NO:
201, and a CDR3 region having SEQ ID NO:
285, or
a CDR1 region having SEQ ID NO:
118, a CDR2 region having SEQ ID NO:
202, and a CDR3 region having SEQ ID NO:
286, or
a CDR1 region having SEQ ID NO:
119, a CDR2 region having SEQ ID NO:
203, and a CDR3 region having SEQ ID NO:
287, or
a CDR1 region having SEQ ID NO:
120, a CDR2 region having SEQ ID NO:
204, and a CDR3 region having SEQ ID NO:
288, or
a CDR1 region having SEQ ID NO:
121, a CDR2 region having SEQ ID NO:
205, and a CDR3 region having SEQ ID NO:
289, or
a CDR1 region having SEQ ID NO:
122, a CDR2 region having SEQ ID NO:
206, and a CDR3 region having SEQ ID NO:
290, or
a CDR1 region having SEQ ID NO:
123, a CDR2 region having SEQ ID NO:
207, and a CDR3 region having SEQ ID NO:
291, or
a CDR1 region having SEQ ID NO:
124, a CDR2 region having SEQ ID NO:
208, and a CDR3 region having SEQ ID NO:
292, or
a CDR1 region having SEQ ID NO:
125, a CDR2 region having SEQ ID NO:
209, and a CDR3 region having SEQ ID NO:
293, or
a CDR1 region having SEQ ID NO:
126, a CDR2 region having SEQ ID NO:
210, and a CDR3 region having SEQ ID NO:
294, or
a CDR1 region having SEQ ID NO:
127, a CDR2 region having SEQ ID NO:
211, and a CDR3 region having SEQ ID NO:
295, or
a CDR1 region having SEQ ID NO:
128, a CDR2 region having SEQ ID NO:
212, and a CDR3 region having SEQ ID NO:
296, or
a CDR1 region having SEQ ID NO:
129, a CDR2 region having SEQ ID NO:
213, and a CDR3 region having SEQ ID NO:
297, or
a CDR1 region having SEQ ID NO:
130, a CDR2 region having SEQ ID NO:
214, and a CDR3 region having SEQ ID NO:
298, or
a CDR1 region having SEQ ID NO:
131, a CDR2 region having SEQ ID NO:
215, and a CDR3 region having SEQ ID NO:
299, or
a CDR1 region having SEQ ID NO:
132, a CDR2 region having SEQ ID NO:
216, and a CDR3 region having SEQ ID NO:
300, or
a CDR1 region having SEQ ID NO:
133, a CDR2 region having SEQ ID NO:
217, and a CDR3 region having SEQ ID NO:
301, or
a CDR1 region having SEQ ID NO:
134, a CDR2 region having SEQ ID NO:
218, and a CDR3 region having SEQ ID NO:
302, or
a CDR1 region having SEQ ID NO:
135, a CDR2 region having SEQ ID NO:
219, and a CDR3 region having SEQ ID NO:
303, or
a CDR1 region having SEQ ID NO:
136, a CDR2 region having SEQ ID NO:
220, and a CDR3 region having SEQ ID NO:
304, or
a CDR1 region having SEQ ID NO:
137, a CDR2 region having SEQ ID NO:
221, and a CDR3 region having SEQ ID NO:
305, or
a CDR1 region having SEQ ID NO:
138, a CDR2 region having SEQ ID NO:
222, and a CDR3 region having the amino acid sequence NRY, or
a CDR1 region having SEQ ID NO:
139, a CDR2 region having SEQ ID NO:
223, and a CDR3 region having SEQ ID NO:
306, or
a CDR1 region having SEQ ID NO:
140, a CDR2 region having SEQ ID NO:
224, and a CDR3 region having SEQ ID NO:
307, or
a CDR1 region having SEQ ID NO:
141, a CDR2 region having SEQ ID NO:
225, and a CDR3 region having SEQ ID NO:
308, or
a CDR1 region having SEQ ID NO:
142, a CDR2 region having SEQ ID NO:
226, and a CDR3 region having SEQ ID NO:
309, or
a CDR1 region having SEQ ID NO:
143, a CDR2 region having SEQ ID NO:
227, and a CDR3 region having SEQ ID NO:
310, or
a CDR1 region having SEQ ID NO:
144, a CDR2 region having SEQ ID NO:
228, and a CDR3 region having SEQ ID NO:
311, or
a CDR1 region having SEQ ID NO:
145, a CDR2 region having SEQ ID NO:
229, and a CDR3 region having SEQ ID NO:
312, or
a CDR1 region having SEQ ID NO:
146, a CDR2 region having SEQ ID NO:
230, and a CDR3 region having SEQ ID NO:
313, or
a CDR1 region having SEQ ID NO:
147, a CDR2 region having SEQ ID NO:
231, and a CDR3 region having SEQ ID NO:
314, or
a CDR1 region having SEQ ID NO:
148, a CDR2 region having SEQ ID NO:
232, and a CDR3 region having SEQ ID NO:
315, or
a CDR1 region having SEQ ID NO:
149, a CDR2 region having SEQ ID NO:
233, and a CDR3 region having SEQ ID NO:
316, or
a CDR1 region having SEQ ID NO:
150, a CDR2 region having SEQ ID NO:
234, and a CDR3 region having SEQ ID NO:
317, or
a CDR1 region having SEQ ID NO:
151, a CDR2 region having SEQ ID NO:
235, and a CDR3 region having SEQ ID NO:
318, or
a CDR1 region having SEQ ID NO:
152, a CDR2 region having SEQ ID NO:
236, and a CDR3 region having SEQ ID NO:
319, or
a CDR1 region having SEQ ID NO:
153, a CDR2 region having SEQ ID NO:
237, and a CDR3 region having SEQ ID NO:
320, or
a CDR1 region having SEQ ID NO:
154, a CDR2 region having SEQ ID NO:
238, and a CDR3 region having SEQ ID NO:
321, or
a CDR1 region having SEQ ID NO:
155, a CDR2 region having SEQ ID NO:
239, and a CDR3 region having SEQ ID NO:
322, or
a CDR1 region having SEQ ID NO:
156, a CDR2 region having SEQ ID NO:
240, and a CDR3 region having SEQ ID NO:
323, or
a CDR1 region having SEQ ID NO:
157, a CDR2 region having SEQ ID NO:
241, and a CDR3 region having SEQ ID NO:
324, or
a CDR1 region having SEQ ID NO:
158, a CDR2 region having SEQ ID NO:
242, and a CDR3 region having SEQ ID NO:
325, or
a CDR1 region having SEQ ID NO:
159, a CDR2 region having SEQ ID NO:
243, and a CDR3 region having SEQ ID NO:
326, or
a CDR1 region having SEQ ID NO:
160, a CDR2 region having SEQ ID NO:
244, and a CDR3 region having SEQ ID NO:
327, or
a CDR1 region having SEQ ID NO:
161, a CDR2 region having SEQ ID NO:
245, and a CDR3 region having SEQ ID NO:
328, or
a CDR1 region having SEQ ID NO:
162, a CDR2 region having SEQ ID NO:
246, and a CDR3 region having SEQ ID NO:
329, or
a CDR1 region having SEQ ID NO:
163, a CDR2 region having SEQ ID NO:
247, and a CDR3 region having SEQ ID NO:
330, or
a CDR1 region having SEQ ID NO:
164, a CDR2 region having SEQ ID NO:
248, and a CDR3 region having SEQ ID NO:
331, or
a CDR1 region having SEQ ID NO:
165, a CDR2 region having SEQ ID NO:
249, and a CDR3 region having SEQ ID NO:
332, or
a CDR1 region having SEQ ID NO:
166, a CDR2 region having SEQ ID NO:
250, and a CDR3 region having SEQ ID NO:
333, or
a CDR1 region having SEQ ID NO:
167, a CDR2 region having SEQ ID NO:
251, and a CDR3 region having SEQ ID NO:
334, or
a CDR1 region having SEQ ID NO:
168, a CDR2 region having SEQ ID NO:
252, and a CDR3 region having SEQ ID NO:
335.

334 more elements

Dependencies are inferred from the claim text.

Facts

Application

17/113,552Filed 7 Dec 2020

Priority

EP 14191959.75 Nov 2014

Published

4 Nov 2021

Term ends

Not estimatedThe source records don’t support an estimate.

Classification11 codes

C12N 15/8282
For biotic stress resistance, pathogen resistance, disease resistance: for fungal resistance
C07K 16/14
Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies: against material from fungi, algea or lichens
C12N 15/8279
For stress resistance, e.g. heavy metal resistance: for biotic stress resistance, pathogen resistance, disease resistance
C07K 2317/22
Characterized by taxonomic origin: from camelids, e.g. camel, llama or dromedary

Transgenic plant comprising a polynucleotide encoding a variable domain of heavy-chain antibody

Life of this patent

Transgenic plant comprising a polynucleotide encoding a variable domain of heavy-chain antibody

Life of this patent

Abstract

Claims26