US 2021/0340554 A1 · METHOD FOR PRODUCING GENOME-MODIFIED PLANTS FROM PLANT PROTOPLASTS AT HIGH EFFICIENCY

Abstract

The present invention relates to a method of increasing the production efficiency of gene-edited plants, regenerated from plant protoplasts, by use of a Cas protein-guide RNA ribonucleoprotein (RNP). According to the present invention, the method of increasing the production efficiency of gene-edited plants makes it possible to efficiently produce target gene-mutated plants and to minimize the introduction of foreign DNA into plants. Thus, the present invention can be very advantageously used in a wide variety of fields, including agriculture, food and biotechnology.

Claims15

Dependent claims

A method for increasing the production efficiency of a genome-edited plant from a plant protoplast, comprising the steps of:

(i) editing a genome of the plant protoplast by a direct introduction of a Cas protein-guide RNA ribonucleoprotein (RNP) in which a Cas protein and a guide RNA in the form of naked RNA are pre-assembled into an isolated plant protoplast without using a vector;
and
(ii) producing a genome-edited plant by regenerating the plant protoplast.

Dependencies are inferred from the claim text.

Facts

Application

17/366,320Filed 2 Jul 2021

Priority

KR 10-2015-01403146 Oct 2015

Published

4 Nov 2021

Term ends

Not estimatedThe source records don’t support an estimate.

Classification9 codes

C12N 15/8213
Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for…:…
C12N 9/222
Ribonucleases [RNase]; Deoxyribonucleases [DNase]: clustered regularly interspaced short palindromic repeats [CRISPR]-associated [CAS] enzymes
C12N 15/11
Recombinant DNA-technology: dNA or RNA fragments; Modified forms thereof ; Non-coding nucleic acids having a biological activity
C12N 15/8206
Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for…:…

Method for producing genome-modified plants from plant protoplasts at high efficiency

Life of this patent

Method for producing genome-modified plants from plant protoplasts at high efficiency

Life of this patent

Abstract

Claims15