Photoactivatable tet expression control system

A kit for a photoactivatable tetracycline gene expression control system, comprising:

• a target gene expression vector including a tetracycline response element having a TetO sequence, a promoter which is positioned downstream of the tetracycline response element and controlled by the tetracycline response element, and a multicloning site which is positioned downstream of the promoter and into which a target gene will be inserted;
• a first expression vector including a first fusion protein expression cassette containing a gene that encodes a first fusion protein in which a Tet repressor protein or a reverse Tet repressor protein is linked to CIB 1 or a variant thereof;
• and
• a second expression vector including a second fusion protein expression cassette containing a gene that encodes a second fusion protein in which a transactivation domain of a transactivation element p65 is linked to Cry2 or a variant thereof.

A kit for a photoactivatable tetracycline gene expression control system, comprising:

• a target gene expression vector including a tetracycline response element having a TetO sequence, a promoter which is positioned downstream of the tetracycline response element and controlled by the tetracycline response element, and a multicloning site which is positioned downstream of the promoter and into which a target gene will be inserted;
• a first expression vector including a first fusion protein expression cassette containing a gene that encodes a first fusion protein in which a Tet repressor protein or a reverse Tet repressor protein is linked to Bphp1 or a variant thereof;
• and
• a second expression vector including a second fusion protein expression cassette containing a gene that encodes a second fusion protein in which a transactivation domain of a transactivation element p65 is linked to Q-PAS1 or a variant thereof.