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US 2021/0340542 A1Published application
Rna molecules, methods of producing circular rna, and treatment methods
AssigneeCornell University
InventorsSamie R. JAFFREY, Jacob L. LITKE
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Abstract
The present invention relates to a RNA molecule comprising a first ribozyme, a first ligation sequence, an effector molecule, a second ligation sequence, and a second ribozyme. Methods of producing circular RNA molecules and treatment methods are also disclosed.
Claims40
A RNA molecule comprising:
- a first ribozyme;
- a first ligation sequence;
- an effector molecule;
- a second ligation sequence;
- and
- a second ribozyme.
3 more elements
A method of producing a circular RNA molecule, said method comprising:
- providing one or more vectors comprising:
- a nucleic acid sequence encoding a 5′ polymerase promoter sequence;
- a nucleic acid sequence encoding a first ribozyme;
- a nucleic acid sequence encoding an effector sequence;
- a nucleic acid sequence encoding a second ribozyme;
- and
- a nucleic acid sequence encoding a 3′ polymerase terminator sequence;
- transcribing the one or more vectors to produce one or more linear RNA molecules;
- and
- contacting the linear RNA molecules with an RNA ligase to circularize the linear RNA molecule thereby producing a circular RNA molecule.
7 more elements
An RNA molecule comprising a 5′-OH end and a 2′,3′-cyclic phosphate end, wherein the RNA molecule further comprises an effector molecule between the 5′-OH and the 2′,3′-cyclic phosphate ends.
Dependencies are inferred from the claim text.