Genetically-encoded bicyclic peptide libraries
AssigneeThe Governors of the University of Alberta
InventorsRatmir DERDA, Raja MUKHERJEE, Vivian TRIANA
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Abstract
The invention relates to a bicyclic peptide complex comprising a peptide construct, said construct comprising (i) a polypeptide with free terminus (N or C); (ii) optionally, a nucleic acid encoding the polypeptide; (iii) a twofold-symmetric linker (TSL) compound attached to said polypeptide where the linker is attached to the terminus of polypeptide via a covalent bond and to at least two discrete side chains of the peptide. The invention also relates to libraries, and to methods for making complexes and to methods of screening using the same.
Claims39
A peptide construct comprising a bicyclic structure resulting from attaching a linker having a first end and a second end comprising two reactive groups to a polypeptide, wherein the first end is ligated to a terminus of the polypeptide by a covalent bond, and wherein both reactive groups of the linker second end are each reacted to side chain residues of the polypeptide.
A method for making a phage display complex, said method comprising (i) providing a phage particle comprising a polypeptide having a terminus (ii) providing a linker having a first end and a second end comprising two reactive groups, and forming an intermediate complex by ligating the linker first end with the polypeptide terminus to form a covalent bond, and (iii) forming a bicyclic structure from the intermediate complex by reacting both reactive groups of the linker second end to side chain residues of the polypeptide.
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Dependencies are inferred from the claim text.