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US 2021/0340518 A1Published application
Method for the production of acrylic acid or salts thereof
AssigneeBASF SE
InventorsDiego Ghislieri, Tobias Joachim Zimmermann, Stefan Seemayer, Michael Breuer, Doreen Schactschabel
Life of this patent
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Abstract
Described herein are methods for the production of ammonium acrylate or salts thereof from acrylonitrile using nitrilase as a catalyst.
Claims19
An isolated nitrilase capable of catalysing a reaction from (meth-) acrylonitrile to (meth-) ammonium acrylate in an aqueous medium comprising water, nitrilase and (meth-) acrylonitrile and/or (meth-) ammonium acrylate wherein the concentration of (meth-) ammonium acrylate in the aqueous medium after an incubation is at least 50% (w/w) and the concentration of (meth-) acrylonitrile is below 0.1% (w/w).
A process for producing (meth-) ammonium acrylate comprising the steps of
- a. Providing an aqueous medium comprising water, one or more nitrilase and (meth-) acrylonitrile,
- b. Incubating the aqueous medium and
- c. Optionally isolating the (meth-) ammonium acrylate from the reaction mixture, wherein the one or more nitrilase is selected from the group consisting of
- i. an amino acid molecule of SEQ ID NO:
- 2, 4, 6, 8, 10, 12, 14, 18, 20, 22, 26, 28, 30, 32, 34, 38, 40, 42, 46, 48, 52, 54, 56, 60, 62, 64, 66 or 68 or a functional fragment thereof,
- ii. an amino acid molecule having at least 55% identity to the amino acid molecule of SEQ ID NO:
- SEQ ID NO:
- 2, 4, 6, 8, 10, 12, 14, 18, 20, 22, 26, 28, 30, 32, 34, 38, 40, 42, 46, 48, 52, 54, 56, 60, 62, 64, 66 or 68 or a functional fragment thereof,
- iii. an amino acid molecule encoded by a nucleic acid molecule of SEQ ID NO 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 25, 27, 29, 31, 33, 37, 39, 41, 45, 47, 51, 53, 55, 59, 61, 63, 65 or 67 or a functional fragment thereof,
- iv. an amino acid molecule encoded by a nucleic acid molecule having at least 70% identity to SEQ ID NO:
- 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 25, 27, 29, 31, 33, 37, 39, 41, 45, 47, 51, 53, 55, 59, 61, 63, 65 or 67 or a functional fragment thereof, and
- v. an amino acid molecule encoded by a nucleic acid molecule hybridizing under stringent conditions to a fragment of at least 250 bases complementary to SEQ ID NO 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 25, 27, 29, 31, 33, 37, 39, 41, 45, 47, 51, 53, 55, 59, 61, 63, 65 or 67 or a functional fragment thereof,
- wherein the amino acid molecule as defined in ii., iv. and v. has an activity of converting (meth-) acrylonitrile to (meth-) ammonium acrylate.
10 more elements
An isolated nitrilase comprising a sequence selected from the group consisting of
- a. an amino acid molecule of SEQ ID NO:
- 2, 4, 6, 8, 10, 12, 14, 18, 20, 22, 26, 28, 30, 32, 34, 38, 40, 42, 46, 48, 52, 54, 56, 60, 62, 64, 66 or 68 or a functional fragment thereof,
- b. an amino acid molecule having at least 55% identity to the amino acid molecule of SEQ ID NO:
- SEQ ID NO:
- 2, 4, 6, 8, 10, 12, 14, 18, 20, 22, 26, 28, 30, 32, 34, 38, 40, 42, 46, 48, 52, 54, 56, 60, 62, 64, 66 or 68 or a functional fragment thereof,
- c. an amino acid molecule encoded by a nucleic acid molecule of SEQ ID NO 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 25, 27, 29, 31, 33, 37, 39, 41, 45, 47, 51, 53, 55, 59, 61, 63, 65 or 67 or a functional fragment thereof,
- d. an amino acid molecule encoded by a nucleic acid molecule having at least 70% identity to SEQ ID NO:
- 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 25, 27, 29, 31, 33, 37, 39, 41, 45, 47, 51, 53, 55, 59, 61, 63, 65 or 67 or a functional fragment thereof, and
- e. an amino acid molecule encoded by a nucleic acid molecule hybridizing under stringent conditions to a fragment of at least 250 bases complementary to SEQ ID NO 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 25, 27, 29, 31, 33, 37, 39, 41, 45, 47, 51, 53, 55, 59, 61, 63, 65 or 67 or a functional fragment thereof,
- wherein the amino acid molecule as defined in b., d. and e. catalyses a reaction from (meth-) acrylonitrile to (meth-) ammonium acrylate in an aqueous medium.
7 more elements
A recombinant construct comprising a nitrilase wherein the nitrilase is selected from the group consisting of
- i. an amino acid molecule of SEQ ID NO:
- 2, 4, 6, 8, 10, 12, 14, 18, 20, 22, 26, 28, 30, 32, 34, 38, 40, 42, 46, 48, 52, 54, 56, 60, 62, 64, 66 or 68 or a functional fragment thereof,
- ii. an amino acid molecule having at least 55% identity to the amino acid molecule of SEQ ID NO:
- SEQ ID NO:
- 2, 4, 6, 8, 10, 12, 14, 18, 20, 22, 26, 28, 30, 32, 34, 38, 40, 42, 46, 48, 52, 54, 56, 60, 62, 64, 66 or 68 or a functional fragment thereof,
- iii. an amino acid molecule encoded by a nucleic acid molecule of SEQ ID NO 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 25, 27, 29, 31, 33, 37, 39, 41, 45, 47, 51, 53, 55, 59, 61, 63, 65 or 67 or a functional fragment thereof,
- iv. an amino acid molecule encoded by a nucleic acid molecule having at least 70% identity to SEQ ID NO:
- 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 25, 27, 29, 31, 33, 37, 39, 41, 45, 47, 51, 53, 55, 59, 61, 63, 65 or 67 or a functional fragment thereof, and
- v. an amino acid molecule encoded by a nucleic acid molecule hybridizing under stringent conditions to a fragment of at least 250 bases complementary to SEQ ID NO 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 25, 27, 29, 31, 33, 37, 39, 41, 45, 47, 51, 53, 55, 59, 61, 63, 65 or 67 or a functional fragment thereof,
- wherein the amino acid molecule as defined in ii., iv. and v. catalyses a reaction from (meth-) acrylonitrile to (meth-) ammonium acrylate in an aqueous medium.
7 more elements
A composition comprising water, a nitrilase, (meth-) acrylonitrile and/or (meth-) ammonium acrylate wherein the nitrilase is selected from the group consisting of
- i. an amino acid molecule of SEQ ID NO:
- 2, 4, 6, 8, 10, 12, 14, 18, 20, 22, 26, 28, 30, 32, 34, 38, 40, 42, 46, 48, 52, 54, 56, 60, 62, 64, 66 or 68 or a functional fragment thereof,
- ii. an amino acid molecule having at least 55% identity to the amino acid molecule of SEQ ID NO:
- SEQ ID NO:
- 2, 4, 6, 8, 10, 12, 14, 18, 20, 22, 26, 28, 30, 32, 34, 38, 40, 42, 46, 48, 52, 54, 56, 60, 62, 64, 66 or 68 or a functional fragment thereof,
- iii. an amino acid molecule encoded by a nucleic acid molecule of SEQ ID NO 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 25, 27, 29, 31, 33, 37, 39, 41, 45, 47, 51, 53, 55, 59, 61, 63, 65 or 67 or a functional fragment thereof,
- iv. an amino acid molecule encoded by a nucleic acid molecule having at least 70% identity to SEQ ID NO:
- 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 25, 27, 29, 31, 33, 37, 39, 41, 45, 47, 51, 53, 55, 59, 61, 63, 65 or 67 or a functional fragment thereof, and
- v. an amino acid molecule encoded by a nucleic acid molecule hybridizing under stringent conditions to a fragment of at least 250 bases complementary to SEQ ID NO 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 25, 27, 29, 31, 33, 37, 39, 41, 45, 47, 51, 53, 55, 59, 61, 63, 65 or 67 or a functional fragment thereof,
- wherein the amino acid molecule as defined in ii., iv. and v. catalyses a reaction from (meth-) acrylonitrile to (meth-) ammonium acrylate in an aqueous medium.
7 more elements
Dependencies are inferred from the claim text.