Modified Filamentous Fungal Host Cell
AssigneeNovozymes A/S
InventorHiroaki Udagawa
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Abstract
The present invention relates to filamentous fungal cells secreting a polypeptide of interest, wherein the expression of a citT gene is altered, reduced or eliminated compared to a non-mutated otherwise isogenic or parent cell, and methods of producing a secreted polypeptide of interest in said cells as well as methods of producing said cells.
Claims24
1-23. (canceled)
A mutated filamentous fungal host cell comprising a heterologous polynucleotide encoding a secreted polypeptide of interest, wherein the expression of a citT gene is reduced or eliminated compared to a non-mutated otherwise isogenic or parent cell, wherein said citT gene encodes a citrate transporter polypeptide, CitT, having at least 80% amino acid sequence identity with the polypeptide of SEQ ID NO:
- 3.
A method of producing a secreted polypeptide of interest, said method comprising the steps of:
- a) cultivating a mutated filamentous fungal host cell comprising a heterologous polynucleotide encoding the secreted polypeptide of interest under conditions conducive to the expression of the secreted polypeptide of interest, wherein the expression of a citT gene is reduced or eliminated compared to a non-mutated otherwise isogenic or parent cell, and wherein said citT gene encodes a citrate transporter polypeptide, CitT, having at least 80% amino acid sequence identity with SEQ ID NO:
- 3;
- and, optionally,
- b) recovering the secreted polypeptide of interest.
A method of producing a mutated filamentous fungal host cell having an improved yield and/or productivity of a secreted heterologous polypeptide of interest, said method comprising the following steps in no particular order:
- a) transforming a filamentous fungal host cell with a heterologous polynucleotide encoding the secreted polypeptide of interest;
- and
- b) mutating the host cell by reducing or eliminating the expression of a citT gene in the filamentous fungal host cell, wherein said citT gene encodes a citrate transporter polypeptide, CitT, having at least 80% amino acid sequence identity with the amino acid sequence shown in SEQ ID NO:
- 3.
Dependencies are inferred from the claim text.