Directed evolution of cyp52a12 gene and its use in dicarboxylic acid production

A vector, comprising an isolated nucleic acid molecule which encoding an isolated modified CYP52A12 protein, wherein the vector is a prokaryotic vector, a virus vector or a eukaryotic vector;

• wherein, with reference to the amino acid numbering of SEQ ID NO:
• 20, the modified CYP52A12 protein comprises Asp at a position corresponding to the position 76 of SEQ ID NO:
• 20 and Ile at a position corresponding to the position 470 of SEQ ID NO:
• 20, compared to an unmodified CYP52A12 protein, and the modified CYP52A12 protein comprises an amino acid sequence having at least about 99% sequence identity with SEQ ID NO:
• 20 and has the capability of producing a long chain dicarboxylic acid under an acidic culture condition that is at least about 95% or more of that of the modified CYP52A12 protein comprising the amino acid sequence set forth in SEQ ID NO:
• 21.