Generation of Post-Mitotic Migratory Cortical Interneurons
OwnerSangmi Chunginventor
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Abstract
The present disclosure provides populations of synchronized post-mitotic migratory cortical interneurons (cINS) derived from pluripotent stem cells and cell culture methods for generating said populations of cINs. The disclosure also provides efficient methods for cryopreservation of the derived cINs.
Claims16
A method of generating MGE progenitors from pluripotent stem cells said method comprising the step of establishing sphere cultures from pluripotent stem cells under spinner culture conditions.
A method of generating a synchronized culture of cINs, derived from pluripotent stem cells, comprising the step of culturing of said cells in the presence of one or more of the following components:
- CultureOne, gamma secretase inhibitor DAPT and CDK4/6 inhibitor PD0332991.
A method of cryopreserving synchronized cultures of cINs wherein said interneurons are cryopreserved in the presence of Trehalose.
Dependencies are inferred from the claim text.