Method for inducing and differentiating pluripotent stem cells and uses thereof

Method for differentiating induced pluripotent stem cells or any embryonic stem like cells into endothelial cells, wherein said method comprises the following steps

• (i) inducing the formation of embryo bodies starting from the induced pluripotent stem cells or embryonic-like cells by:
• (ia) plating the cells in a medium specific for embryo bodies at a concentration ranging from 5 to 50, colonies/plate to obtain embryo bodies formation;
• (ib) after about 48 h from step (ia), culturing the obtained embryo bodies in suspension in the medium specific for embryo bodies further comprising BMP4 at a concentration ranging from 5 to 40 mg/ml;
• (ic) after about 90-100 hours from step (ia) further adding to the medium FGF specific for embryo bodies at a concentration ranging from 5 to 40 ng/ml;
• (id) after about 130-150 hours from step (ia) seeding the obtained embryo bodies on a gelatin coated plate in a medium specific for embryo bodies comprising:
• FGF at a concentration ranging from 5 to 40 ng/ml;
• and/or VEGF at a concentration ranging from 30 to 70 ng/ml;
• (ie) after about 180-200 hours from step (ia) replacing the medium specific for embryo bodies with a medium including VEGF at a concentration 30-70 ng/ml until the end of culturing 20 days;
• (ii) collecting the culture cells wherein said cultured cells are endothelial cells.