Loading patent…
AssigneeCenter for Excellence In Molecular Cell Science, Chinese Academy of Sciences
InventorsLijian Hui, Ludi Zhang, Kun Zhang
Estimating the term…
Provided are a method for expanding a hepatocyte in vitro and an application thereof. A culture system is provided for reprogramming a human hepatocyte into a proliferating intermediate-state cell between a mature hepatocyte and a liver progenitor cell. The liver repopulation ability of the system was verified in animals. The method does not require the introduction of an exogenous gene into a hepatocyte, and the expansion of the hepatocyte can be realized by conventional culture. The obtained hepatocyte can be passaged, and can be cultured to maturation to obtain a functional mature human hepatocyte.
A method for in vitro hepatocyte expansion, comprising culturing hepatocytes in a cell culture medium containing a Wnt signaling pathway agonist to expand the hepatocytes in vitro.
(canceled)
A culture medium for in vitro expansion of hepatocytes, comprising Wnt signaling pathway agonist, N-acetylcysteine, nicotinamide, FGF10, EGF, HGF, [Leu15]-gastrin I, A 83-01, and Y-27632, wherein the Wnt signaling pathway agonist is selected from the group consisting of Wnt3a protein, Wnt3a conditioned medium, and CHIR, or a combination thereof.
(canceled)
Dependencies are inferred from the claim text.