System and Method for Centralized Fluid Management and Culture Control

A method for producing a plurality of independent tissue constructs simultaneously in a bioreactor system, the bioreactor system including a plurality of bioreactors, at least one waste line conducting waste from the plurality of bioreactors, at least one media line conducting a media towards the plurality of bioreactors, the at least one media line, the at least one waste line, and the plurality of bioreactors being coupled by a fluid path, the method comprising:

• fluidically isolating each of the plurality of bioreactors from each other and from other components of the bioreactor system, each of the plurality of bioreactors producing one of the plurality of independent tissue constructs;
• and
• feeding the plurality of independent tissue constructs with the media conducted from a central media reservoir through the at least one media line to the plurality of independent tissue constructs.

A system for alternating a direction of a fluid flow of a fluid entering, exiting, or both entering and exiting a pumping cassette without changing the direction of the fluid flow past at least one on-cassette inline sensor and at least one gas exchange surface, the system comprising:

• a pumping chamber including a pumping chamber cassette membrane, the pumping chamber being associated with a corresponding pumping valve;
• and
• a fluidic pathway analogous to an h-bridge enabling applying pneumatic pressure to the pumping chamber cassette membrane, the corresponding pumping valve being open, the pneumatic pressure enabling pumping the fluid in a first flow path past the at least one on-cassette inline sensor if a first valve is open, pumping the fluid into a gas exchange zone if a second valve is open, and pumping the fluid into a sample line if a third valve is open, the fluidic pathway enabling isolating the pumping chamber by closing a valve corresponding to a second flow path by blocking the pumped fluid in the second flow path, the second flow path traveling in an opposite direction to the first flow path, the fluidic pathway enabling routing the fluid to the pumping chamber using a fourth valve or a fifth valve, the fluidic pathway enabling tying the first valve and the fourth valve together, and the second valve and the fifth valve together.