Device for a cell suspension culture

A device for a cell suspension culture comprising:

• a culture chamber comprising a first wall and a second wall delimiting an inner compartment, said first and second walls having an axisymmetric shape and being connected to each other in order to form an inner continuous surface;
• an inlet opening provided in the first wall and suitable to enable a fluid to enter the culture chamber;
• a supply conduit connected to the inlet opening and suitable to enable to supply the fluid towards the culture chamber through the inlet opening;
• a valve member arranged inside the culture chamber at the inlet opening, displaceable between an open position, wherein the valve member is distanced from the inlet opening and enables the outflow of the fluid inside the culture chamber, and a dosed position wherein the valve member tightly doses the inlet opening;
• and
• a base body inside which one of the first and second walls is arranged, and a lid suitable to be tightly connected to the base body and in which the other one of the first and second walls of the culture chamber is arranged;
• wherein the first wall comprises a bottom zone and a joint zone suitable to connect to each other the bottom zone and the second wall, wherein the joint zone has an arc-of-a-circle cross-section having a concavity facing the inside of the culture chamber;
• wherein the second wall comprises a central zone and an annular zone with an arc-of-a-circle cross-section having a concavity facing the inside of the culture chamber;
• wherein the second wall comprises a central zone and an annular zone with an arc-of-a-circle cross-section having a concavity facing the inside of the culture chamber;
• and
• wherein in the open position, the valve member opens a port for the passage of the fluid, with a predetermined height with respect to the first wall

A device for a cell suspension culture comprising:

• a culture chamber comprising a first wall and a second wall delimiting an inner compartment, said first and second walls having an axisymmetric shape and being connected to each other in order to form an inner continuous surface;
• an inlet opening provided in the first wall and suitable to enable a fluid to enter the culture chamber;
• a supply conduit connected to the inlet opening and suitable to enable to supply the fluid towards the culture chamber through the inlet opening;
• a valve member arranged inside the culture chamber at the inlet opening, displaceable between an open position, wherein the valve member is distanced from the inlet opening and enables the outflow of the fluid inside the culture chamber, and a dosed position wherein the valve member tightly doses the inlet opening;
• a movement device for moving the valve member, suitable to cause the valve member to be displaced between the open position and the dosed position, said movement device being disposed outside the culture chamber;
• and
• an outlet opening for enabling the fluid to exit the culture chamber, said outlet opening being arranged in the second wall;
• wherein the first wall comprises a bottom zone and a joint zone suitable to connect to each other the bottom zone and the second wall, wherein the joint zone has an arc-of-a-circle cross-section having a concavity facing the inside of the culture chamber;
• wherein the second wall comprises a central zone and an annular zone with an arc-of-a-circle cross-section having a concavity facing the inside of the culture chamber;
• wherein in the open position, the valve member opens a port for the passage of the fluid, with a predetermined height with respect to the first wall;
• wherein the cross-section of the joint zone of the first wall has a curve radius comprised between five and twenty times the height of the port of the fluid passage;
• wherein the cross-section of the annular zone of the second wall has a curve radius comprised between three and ten times the height of the port for the passage of the fluid, and is less than the curve radius of the joining zone of the first wall;
• and
• wherein the movement device of the valve member comprises a magnetic element, the valve member being provided with a magnetic counter-element suitable to interact with said magnetic element.