Apparatus for cell culture and method for cell culture using the same

Abstract

The present disclosure provides a three-dimensional cell culture method and a cell culture apparatus using the same. The cell culture method includes lowering, when culturing cells on at least one surface of a porous support, a concentration of a cell-secreted substance in a lower region of the other surface of the porous support. The cell culture apparatus includes: an upper chamber including an opening, porous support having at least one surface on which cells are cultured, and a cell culture space in which a culture medium is housed; a lower chamber including a space in which at least one upper chamber is disposed; and a device lowering a concentration of a cell-secreted substance in a lower region of the other surface of the porous support.

Dependencies are inferred from the claim text.