Pretreatment apparatus and pretreatment method

A sample pretreatment method comprising:

• centrifuge a sample in a container by rotating a holder holding the container under a temperature adjusted by a temperature adjustment device in a housing;
• open a port of the housing by moving a shutter;
• dispense a reagent into the container by entering a nozzle through the port;
• and
• close the port by moving the shutter.

A pretreatment apparatus for performing pretreatment of a sample comprising:

• a centrifugal separation device having a holder to hold a container containing the sample and configured to centrifuge the sample by rotating the container on the holder;
• a housing accommodating the centrifugal separation device inside;
• a temperature adjustment device configured to adjust a temperature in the housing under control of the controller;
• a reagent storage including a first reagent storage and a second reagent storage, the first reagent storage storing a first reagent to be dispensed to the container and the second reagent storage storing a second reagent, different from the first reagent, to be dispensed to the container;
• a reagent temperature adjustment device configured to adjust temperatures in the first and second reagent storages at different temperatures.