Methods and systems for controlling oxidative stress

A method for measuring a degree of blood oxidative stress in a subject, comprising:

• collecting a first sample of blood from the subject;
• collecting a second sample of blood from the subject;
• wherein each of the first and second samples of blood contains plasma;
• adding a first agent to the second sample, the first agent being configured to induce reactive oxygen species (ROS) in blood cells of the second sample;
• reacting the second sample after the first agent has been added to the second sample for a first predetermined time period, the first predetermined time period being sufficient to induce production of ROS in blood cells of the second sample;
• reacting the first sample of blood for the first predetermined time period during a first reacting step;
• diluting the first and second samples following the reacting steps to reducing a concentration of ROS in the plasma of each of the first and second samples;
• reacting each of the first and second samples, after dilution, for a second predetermined time period during a second reacting step;
• isolating the plasma of each of the first and second samples following the second reacting step;
• adding a second agent to each of the first and second samples following the isolating step, wherein adding the second agent at least partially reacts with ROS present in the plasma of each of the first and second samples;
• measuring a level in each of the first and second samples indicative of reaction product formed in the plasma after the second agent has reacted with ROS in each of the first and second samples;
• and
• comparing the level of reaction product of the first sample to the level of reaction product of the second sample;
• wherein the comparison step is indicative of the degree of blood oxidative stress in a subject.