AUTOMATIC SAMPLE WORKFLOW FOR LC-MS BASED HbA1c MEASUREMENT ON INTACT PROTEIN LEVEL

A kit comprising

• (i) enrichment means for enriching intact glycated HbA1c molecules, in particular comprising a solid phase having a hydrophobic surface for selective binding of intact glycated HbA1c molecules,
• (ii) a substantially neutral solid phase binding medium for use in diluting a hemolysed whole blood sample and binding sample constituents to the solid phase (i) and
• (iii) an elution medium comprising an organic water-miscible solvent such as acetonitrile for use in eluting sample components from the enrichment means (i).

A diagnostic system for determining glycated hemoglobin A (HbAc1) in a sample comprising

• (i) at least one station for the preparation of samples comprising intact HbA1c molecules, wherein the preparation comprises hemolysis of a whole blood sample,
• (ii) optionally at least one enrichment station adapted for selectively enriching intact hemoglobin A molecule from the sample, and
• (iii) at least one station adapted for the analysis of intact hemoglobin A molecules by mass-spectrometry (MS) and determining the amount or concentration of HbAc1 in the sample.