Detection of phosphokinase signatures
AssigneeThe Regents of the University of California
InventorsJean-Philippe COPPE, Laura J. VAN'T VEER
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Abstract
The disclosure provides methods and compositions for determining the kinase activity profile of a biological sample, e.g., that contains multiple kinases.
Claims36
A method of determining the phosphorylation activity profile of a sample comprising one or more kinases, the method comprising:
- incubating the sample with a panel of sensor peptides comprising a diversity of biological sensor peptides, wherein each biological sensor peptide comprises a substrate region phosphorylated by a kinase, and the diversity of biological sensor peptides comprises biological sensor peptides for different kinases;
- and further, wherein members of the panel of peptides are distributed into separate reaction mixtures to assess phosphorylation activity, such that each reaction mixture represents one biological sensor peptide;
- and
- measuring phosphorylation activity for each peptide in the separate reaction mixtures, thereby determining the phosphorylation activity profile of the sample.
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A panel comprising a plurality of biological sensor peptides to evaluate phosphorylation activity of two or more different kinases.
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A panel of biological sensor peptides to evaluate phosphorylation activity, wherein the panel comprises at least four biological sensor peptides for each of kinase families ABL, AKT, HER, MAPK and SFK, optionally, wherein the panel of sensor peptides comprises at least four biological sensor peptides for each of kinase subfamilies ABL1, AKT1, EGFR, MAPK1/ERK2, MAPK14/p38a, HCK, and SRC.
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Dependencies are inferred from the claim text.