Base material for producing sensor for analysis of detection target, sensor for analysis of detection target, and method for analyzing detection target

A manufacture method of a base material for producing a sensor for analysis of a detection target, comprising:

• a monomolecular film formation step (1-1) of forming on a base material a monomolecular film having a polynucleotide group for nucleic acid aptamer's binding and a polymerization initiating group on a surface thereof;
• a template introduction step (1-2) of introducing a template having on a surface thereof a polynucleotide group capable of complementary binding with the polynucleotide group for nucleic acid aptamer's binding, into the polynucleotide group for nucleic acid aptamer's binding;
• a surface modification step (1-3) of modifying a surface of the template with a polymerizable functional group via a reversible linked group;
• a polymerization step (1-4) of forming a polymer film on a surface of the base material by adding a polymerizable monomer and synthesizing a molecularly imprinted polymer corresponding to a part of the surface of the template using the polymerizable monomer and the polymerizable functional group as substrates and the polymerization initiating group as a polymerization initiator;
• and
• a removal step (1-5) of cleaving the complementary binding and the reversible linked group to convert respectively into a polynucleotide group for nucleic acid aptamer's binding and a group for signal substance's binding, and removing the template.