Methods for non-invasive detection of transplant health or rejection
AssigneeAgilent Technologies, Inc.
InventorsDirk GOOSSENS, Joachim DE SCHRIJVER, Jurgen DEL FAVERO
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Abstract
The present invention relates to the technical field of organ and tissue transplantation and specifically to methods allowing rejection of transplanted organs and tissues by a transplant patient. The present invention provides methods for non-invasive and ex vivo or in vitro detection of the presence or absence of transplant rejection, i.e. transplant health or rejection, The present methods allow for effective and continuous monitoring of transplant status with a minimal or absent discomfort for a transplant patent.
Claims16
1-13. (canceled)
A method for amplifying at least one sample from a recipient of a tissue or organ from a donor, comprising
- (a) amplifying amplicons by nucleic acid amplification of cell-free DNA (cfDNA) wherein each amplicon comprises a Single Nucleotide Polymorphism (SNP), thereby providing amplified amplicons wherein said amplification is performed on:
- (i) at least one sample A of said recipient and at least one sample of said donor;
- or
- (ii) at least one sample A of said recipient;
- (b) determining donor discriminating amplicons;
- (c) amplifying cfDNA from a sample B of said recipient;
- and
- (d) determining the presence or amount of donor derived alleles in said amplified cfDNA.
Dependencies are inferred from the claim text.