US 2021/0332433 A1 · Method for identifying genetic sex of Portunus trituberculatus

Abstract

A method for identifying the genetic sex of Portunus trituberculatus is provided. The SNP markers provided by the present invention presents biallelic heterozygosity in male individuals, but presents homozygous allele type in female individuals, and can be detected by PCR amplification and sequencing method, HRM method or KASP method. The detection method is flexible and the results are stable. Among them, the KASP and HRM detection methods have high throughput, fast detection speed, and simple application. It provides an important tool for the breeding of all-female population of P. trituberculatus.

Claims20

Dependent claims

A Single nucleotide polymorphism (SNP) markers for genetic sex identification of Portunus trituberculatus, comprising a first SNP site of a fragment of SEQ ID NO:1;

or by substituting, deleting, or adding one or more nucleotides from the SEQ ID NO:1;
wherein the SNP site is a complementary fragment of the fragment SEQ ID NO:1, position 1452, which is 1452A>G;
Or
a second SNP sites comprising six sex-linked SNP sites distributed on the fragment of SEQ ID NO:
7, comprising 153C>T, 185G>A, 214T>C, 236T>(A, C), 251T>C, 261A>T;
Or
a third SNP site comprising a sex-linked SNP site distributed at position 878 of SEQ ID NO:
10, and its base is T>C.

5 more elements

Dependencies are inferred from the claim text.

Facts

Application

17/194,252Filed 6 Mar 2021

Priority

CN 202010152999.76 Mar 2020

Published

28 Oct 2021

Term ends

Not estimatedThe source records don’t support an estimate.

Classification4 codes

C12Q 1/6879
Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes: for sex determination
C12Q 1/6858
Nucleic acid amplification reactions: allele-specific amplification
C12Q 2600/156
Oligonucleotides characterized by their use: polymorphic or mutational markers
C12Q
Measuring or testing processes involving enzymes, nucleic acids or microorganisms

Method for identifying genetic sex of Portunus trituberculatus

Life of this patent

Method for identifying genetic sex of Portunus trituberculatus

Life of this patent

Abstract

Claims20