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US 2021/0332431 A1Published application
Methods for nucleic acid sequencing
AssigneeVeracyte, Inc.
InventorsJonathan I. WILDE, Sharlene VELICHKO, Catalin BARBACIORU, James DIGGANS, Giulia C. KENNEDY
Life of this patent
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Abstract
The present disclosure provides methods for sequencing and analysis of nucleic acids.
Claims25
A method for nucleic acid sequencing, comprising
- (a) obtaining a nucleic acid sample, wherein said nucleic acid sample comprises a plurality of messenger ribonucleic acid molecules;
- (b) subjecting said plurality of messenger ribonucleic acid molecules to reverse transcription to yield a plurality of complementary deoxyribonucleic acid molecules;
- and
- (c) subjecting said plurality of complementary deoxyribonucleic acid molecules or derivatives thereof to sequencing.
1 more element
A method for determining that a subject is at risk for usual interstitial pneumonia or non-usual interstitial pneumonia, comprising:
- (a) obtaining a biological sample of said subject,
- (b) assaying nucleic acid molecules derived from said biological sample to identify an expression level of said nucleic acid molecules;
- and,
- (c) processing said expression level to generate a classification of said biological sample as being at risk for said usual interstitial pneumonia or said non-usual interstitial pneumonia.
1 more element
Dependencies are inferred from the claim text.