Serum-free cell culture medium
AssigneeRegeneron Pharmaceuticals, Inc.
InventorsShadia Abike OSHODI, Amy S. JOHNSON, Shawn M. LAWRENCE
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Abstract
The specification describes an improved serum-free animal cell culture medium, which can used for the production of a protein of interest. Ornithine, or a combination of ornithine and putrescine can be added to serum-free media or chemically defined media to improve viable cell density, to reduce cell doubling time, and to increase the production of a protein of interest.
Claims30
A method for cultivating a CHO cell expressing a receptor-Fc-fusion protein, comprising:
- (a) culturing the CHO cell in a cell culture medium at a temperature of about 35° C. to 38° C. during a growth phase to form a cell culture, wherein said cell culture medium is serum-free and comprises plant hydrolysates and polyamines;
- and
- (b) culturing the cell culture in the cell culture medium at a temperature of about 29° C. to 37° C. during a production phase.
A method for cultivating a CHO cell expressing a VEGF trap protein, comprising:
- (a) culturing the CHO cell in a cell culture medium at a temperature of about 35° C. to 38° C. during a growth phase to form a cell culture, wherein said cell culture medium is serum-free and comprises plant hydrolysates and polyamines;
- and
- (b) culturing the cell culture in the cell culture medium at a temperature of about 29° C. to 37° C. during a production phase.
A method for cultivating a CHO cell expressing aflibercept, comprising:
- (a) culturing the CHO cell in a cell culture medium at a temperature of about 35° C. to 38° C. during a growth phase to form a cell culture, wherein said cell culture medium is serum-free and comprises plant hydrolysates and polyamines;
- and
- (b) culturing the cell culture in the cell culture medium at a temperature of about 29° C. to 37° C. during a production phase.
Dependencies are inferred from the claim text.