Method for enhancing extracellular vesicle production
AssigneesResearch Foundation of the City University of New York, Japanese Foundation for Cancer Research
InventorsHiroshi Matsui, Kiyotaka Shiba, Min A. Kang
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Abstract
A method for enhancing extracellular vesicle production is described. A peptide that induces polymer formation is incubated with a cell culture which results in enhanced EV production. The peptide penetrates the cells and subsequently polymerizes upon exposure to enzymes (e.g. phosphatase) within the cell. The cells that contain the newly formed polymers have an increased production of EVs. These EVs can be harvested using centrifugation techniques.
Claims7
A method of enhanced extracellular vesicle production, the method comprising sequential steps of:
- treating the plurality of cells with NapFFK(NDB)Yp at a concentration of at least 5 μM and less than 50 μM, wherein the NapFFK(NDB)Yp had been purified to a purity of at least 93%;
- permitting, for under 30 minutes, the NapFFK(NDB)Yp to polymerize within cells in the plurality of cells due to an enzyme within the cells to form a modified cell culture;
- and
- isolating a first quantity of extracellular vesicles from the modified cell culture, wherein the first quantity of extracellular vesicles is greater than a second quantity of extracellular vesicles isolated from a corresponding plurality of cells that was not treated with the NapFFK(NDB)Yp.
A method of enhanced extracellular vesicle production, the method comprising sequential steps of:
- treating the plurality of cells with NapFFK(NDB)Yp at a concentration of at least 5 μM and less than 50 μM, wherein the NapFFK(NDB)Yp had been purified to a purity of at least 93%;
- permitting, for between 20 minutes and 60 minutes, the NapFFK(NDB)Yp to polymerize within cells in the plurality of cells due to a phosphatase enzyme within the cells to form a modified cell culture;
- and
- isolating a first quantity of extracellular vesicles from the modified cell culture, wherein the first quantity of extracellular vesicles is greater than a second quantity of extracellular vesicles isolated from a corresponding plurality of cells that was not treated with the NapFFK(NDB)Yp;
- wherein the step of isolating occurs between 20 minutes and 60 minutes of the step of treating.
Dependencies are inferred from the claim text.