Method for preparing natural delta-decalactone and delta-dodecalactone by bioreduction of massoia oil

A method for preparing natural delta-decalactone and natural delta-dodecalactone by bioreduction of Massoia Oil, comprising:

• (1) culturing Saccharomyces pastorianus OMK-70 on a solid slant culture medium and a seed culture medium in sequence to obtain a seed culture solution, the Saccharomyces pastorianus OMK-70 was deposited in China Center for Type Culture Collection on Dec. 27, 2019, with a deposit number of CCTCC NO:
• M 20191123;
• (2) inoculating the seed culture solution into a fermentation medium and cultivating, adding Massoia oil in batches after activated strains grow to a logarithmic stage, and continuously adding glucose and continually fermenting;
• (3) adding butyl acetate into a culture solution obtained in step (2), completely stirring and reacting, leaving to stand to generate layers, and then acquiring an upper oil phase;
• and
• (4) recycling the butyl acetate in the upper oil phase, rectifying a residue by controlling a rectification temperature, and collecting fractions at different temperatures to obtain the natural delta-decalactone and the natural delta-dodecalactone.